Orb866958-48 | Fish GnRH ELISA Kit Clinisciences

Fish GnRH ELISA Kit

Catalog Number	orb866958
Category	Assays and Kits
Description	Sandwich Fish GnRH ELISA Kit
Tested applications	ELISA
Reactivity	Fish
Range	0.25-16 ng/mL
Concentration	16 ng/mL
Sensitivity	0.1 ng/mL
Protein Length	2h
Alternative names	GNRH1 ELISA Kit, GRH ELISA Kit, LNRH ELISA Kit, LH
Note	For research use only
Application notes	Standard: 16 ng/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GnRH in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.