K2124-100 assays | Fructose Colorimetric/Fluorometric Assay Kit Clinisciences

Background

Fructose is a ketonic monosaccharide found in many plants and foods. Fructose, along with glucose and galactose, are three dietary monosaccharides that can be absorbed directly into the bloodstream during digestion. Fructose is the sweetest naturally occurring sugar.

The Fructose Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of fructose in cell or tissue culture supernatants based on colorimetric and fluorometric method. In the assay, free fructose is enzymatically converted to β-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to yield fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The kit is suited for high throughput assay of D-fructose.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 1 hour; The sensitive kit contains all necessary reagents for accurate measurement of fructose.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Related Biological Data

Fructose Colorimetric/Fluorometric Assay Kit

Product Citation

1. Zhao S, Gong Z, et al. "Deoxycholic Acid Triggers NLRP3 Inflammasome Activation and Aggravates DSS-Induced Colitis in Mice." Front Immunol. 2016 Nov 28;7:536. PMID:27965665

Storage

Store at -20°C.

Overview

Kit components

Fructose Assay Buffer, OxiRed Probe (in DMSO), Enzyme Mix, Fructose Converting Enzyme, Fructose Standard (100 mM)

Fructose Colorimetric/Fluorometric Assay Kit

Cat# K2124-100 assays

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Marca : ApexBio Technology