Plasmid midiPREP BioSeparation™ Kit

The Plasmid midiPREP BioSeparation™ Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid midiPREP BioSeparation™ Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP BioSeparation™ Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.

Up to 100ml bacterial cells

Up to 250ug of plasmid

<0.003EU/ug

The purified plasmid DNA can be immediately used in any downstream molecular biology application.
* Transfecttion * Microinjection * Sequencing * PCR * Restriction enzyme digestion

Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, thus minimizing the exposure to and disposal of hazardous materials.
Time saving: Complete the process in less than 40 minutes.

Buffer M1, 85ml
Buffer M2, 85ml
Buffer M3, 125ml
Buffer W1, 125ml, 40ml
Buffer W2, 25ml X 2
*Add 100 ml of the ethanol (96~100%) to each bottle of the Buffer W2 before use.
Buffer E, 50ml
RNase A (50mg/ml), 200ul
MD Column, 20 pcs

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards