Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunofluorescence. Immunoblotting: One single band is reactive corresponding to Actin. Working Dilution: 1/5-1/10. Dilute immediately before use with PBS or TBS. Recommended Positive Control: Muscle or myofibrils.
Reactivity
Chicken, Human, Mouse, Rat
Antibody Host
Mouse
Isotype
IgM
Clonality
Monoclonal
Immunogen
Antigen isolated from Chicken skeletal muscle cells
Specificity
This Monoclonal antibody 647 is useful for studying the intracellular distribution and structure of Actin in the cytoskeletal system.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
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