AIF1 / Iba1 (Microglia Marker) (rAIF1/1909), 0.2mg/mL
Cat# BNUB2346-500
Size : 500uL
Marca : Biotium
AIF1 / Iba1 (Microglia Marker) (rAIF1/1909), 0.2mg/mL
Formalin-fixed paraffin-embedded Human Tonsil stained with AIF1 / Iba1 Mouse Recombinant Monoclonal Antibody (rAIF1/1909).
SDS-PAGE Analysis Purified AIF1 / Iba1 Mouse Recombinant Monoclonal Antibody (rAIF1/1909). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing >19000 full-length human proteins using AIF1 Mouse Monoclonal Antibody (rAIF/1909) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
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CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Allograft Inflammatory Factor 1 / IBA1 Recombinant Monoclonal Mouse Antibody (rAIF1/1909)
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Formalin-fixed paraffin-embedded Human Tonsil stained with AIF1 / Iba1 Mouse Recombinant Monoclonal Antibody (rAIF1/1909).
SDS-PAGE Analysis Purified AIF1 / Iba1 Mouse Recombinant Monoclonal Antibody (rAIF1/1909). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing >19000 full-length human proteins using AIF1 Mouse Monoclonal Antibody (rAIF/1909) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.View Spectra Viewer
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Product Description
Allograft Inflammatory Factor 1 (also known as AIF1 or IBA1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email [email protected] to inquire about stock status and lead times before placing your order.
Catalog number key for antibody number 2346, Anti-AIF1 / Iba1 (rAIF1/1909)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
Technology
Product Attributes
research-areas
Immunology
Antibody number
#2346
Size
50 uL, 100 uL, 250 uL, 500 uL
Antibody reactivity (target)
Allograft Inflammatory Factor 1
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal, Recombinant Monoclonal
Clone
rAIF1/1909
Isotype
IgG1, kappa
Molecular weight
17 kDa
Synonyms
Allograft inflammatory factor 1; AIF1; IBA1
Human gene symbol
AIF1
Entrez gene ID
199
SwissProt
P55008
Unigene
76364
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Antibody target cellular localization
Cytoplasmic
Verified antibody applications
IHC (FFPE) (verified)
Species reactivity
Human
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user
Positive control
Lymph Node, Tonsil or Kidney
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array
Monospecific
Antibody research areas
Immunology
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Microglia, Monocytes/macrophages
Documents, Protocols, SDS and COA
Datasheet
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