Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Aloin [1415-73-2]

Stampa

Cat# HY-N0123-1ml

Size : 10mM/1mL

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : MedChemExpress

Contatta il distributore locale :

Telefono : +1 850 650 7790

Aloin (Aloin-A; Barbaloin-A) is a natural anti-tumor anthraquinone glycoside with iron chelating activity. Aloin induces the differentiation of MC3T3-E1 cells into osteoblasts through MAPK-mediated Wnt and Bmp signaling pathways. Alkaline phosphatase (ALP) is an early marker of osteoblast differentiation, and the activity of ALP is also enhanced by Aloin. Aloin also reduces brain edema, reduces blood-brain barrier disruption and improves cortical impact injuries. Aloin is used in research into osteoporosis and traumatic brain injury (TBI).

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Aloin Chemical Structure

CAS No. : 1415-73-2

This product is a controlled substance and not for sale in your territory.

Based on 7 publication(s) in Google Scholar

Other Forms of Aloin:

Aloin B In-stock

Description

In Vitro

Aloin (0.01-1 μM; 10 d) enhances the expression of osteoblast differentiation genes, Bmp-2, Runx2, and collagen 1a in a dose-dependent manner^[3].
Aloin (0.05 μM; 10 d) induces the differentiation of MC3T3-E1 cells into osteoblasts through MAPK-mediated Wnt and Bmp signaling pathways^[3].
Aloin (0.05 μM; 10 d) increases ALP activity in MC3T3-E1 cells^[3].
Aloin (10-80 μg/mL; 4.5 h) increases cell viability after biaxial stretch injury (SI), via attenuates intracellular ROS generation, inhibits changes of mitochondrial membrane potentials in mouse cerebrovascular endothelial cells (ECs)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Aloin (10-30 mg/kg; sinlge dose; ip 30 min before TBI injury) ameliorates traumatic brain injury (TBI) induced brain edema by controlled cortical impact injury in mice. Aloin exhibits antioxidant stress and anti-apoptotic properties in mouse brain capillary endothelial cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

418.39

Formule

C₂₁H₂₂O₉

CAS No.

1415-73-2

Unlabeled Cas

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(C=CC=C2O)[C@H]([C@]3(19)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C4=CC(CO)=CC(O)=C14

Structure Classification

Quinones
Anthraquinones

Phenols
Polyphenols

Initial Source

Plants
Liliaceae
Aloe vera (L.) Burm. f.

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 125 mg/mL (298.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3901 mL	11.9506 mL	23.9011 mL
5 mM	0.4780 mL	2.3901 mL	4.7802 mL
10 mM	0.2390 mL	1.1951 mL	2.3901 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 98.32%

Select Batch:

Fiche technique (284 KB) SDS (394 KB)

COA (260 KB) HNMR (217 KB) LCMS (120 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Pan Q, et al. Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo. Cancer Cell Int. 2013 Jul 12;13(1):69. [Content Brief]

[2]. Tabolacci C, et al. Aloin enhances cisplatin antineoplastic activity in B16-F10 melanoma cells by transglutaminase-induced differentiation. Amino Acids. 2013 Jan;44(1):293-300. [Content Brief]

[3]. Cui Y, et al. Aloin protects against chronic alcoholic liver injury via attenuating lipid accumulation, oxidative stress and inflammation in mice. Arch Pharm Res. 2014 Dec;37(12):1624-33. [Content Brief]

[4]. Pengjam Y, et al. Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling. Biomol Ther (Seoul). 2016 Mar 1;24(2):123-3 [Content Brief]

[5]. Jing Y, et al. Aloin Protects Against Blood-Brain Barrier Damage After Traumatic Brain Injury in Mice. Neurosci Bull. 2020 Jun;36(6):625-638. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3901 mL	11.9506 mL	23.9011 mL	59.7529 mL
	5 mM	0.4780 mL	2.3901 mL	4.7802 mL	11.9506 mL
	10 mM	0.2390 mL	1.1951 mL	2.3901 mL	5.9753 mL
	15 mM	0.1593 mL	0.7967 mL	1.5934 mL	3.9835 mL
	20 mM	0.1195 mL	0.5975 mL	1.1951 mL	2.9876 mL
	25 mM	0.0956 mL	0.4780 mL	0.9560 mL	2.3901 mL
	30 mM	0.0797 mL	0.3984 mL	0.7967 mL	1.9918 mL
	40 mM	0.0598 mL	0.2988 mL	0.5975 mL	1.4938 mL
	50 mM	0.0478 mL	0.2390 mL	0.4780 mL	1.1951 mL
	60 mM	0.0398 mL	0.1992 mL	0.3984 mL	0.9959 mL
	80 mM	0.0299 mL	0.1494 mL	0.2988 mL	0.7469 mL
	100 mM	0.0239 mL	0.1195 mL	0.2390 mL	0.5975 mL

Aloin [1415-73-2]

Cat# HY-N0123-1ml

Contatta il distributore locale :

Marca : MedChemExpress

Contatta il distributore locale :

Other Forms of Aloin:

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Aloin Related Classifications

Keywords:

Si può anche essere interessati nei seguenti prodotti:

Aloin [1415-73-2]

Cat# HY-N0123-1ml

Contatta il distributore locale :

Marca : MedChemExpress

Contatta il distributore locale :

Other Forms of Aloin:

Aloin Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Aloin Related Classifications

Keywords:

Si può anche essere interessati nei seguenti prodotti: