Arginase 1 antibody
Cat# GTX109242-100ul
Size : 100ul
Marca : Genetex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA |
Reactivity | Human, Mouse, Rat |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | 1:100-1:1000 |
FACS | 1:50-1:200 |
IP | 1:100-1:1000 |
ELISA | 1:1000-1:10000 |
Sandwich ELISA | Assay dependent |
Note : Sandwich ELISACapture : GTX634218 / GTX109242, Detection : GTX109242 / GTX634218 |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX109242 WB ImageNon-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX109242 WB ImageNon-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX109242 ELISA ImageSandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
GTX109242 IHC-P ImageArginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX109242 IP Image Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242). |
GTX109242 IHC-Fr ImageArginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920). |
GTX109242 FACS Image Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis. |
GTX109242 ELISA ImageSandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody. |
GTX109242 ICC/IF ImageArginase 1 antibody detects Arginase 1 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm. |
GTX109242 WB ImageVarious tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX109242 WB ImageVarious whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
GTX109242 WB ImageThe data was published in the journal J Neuroinflammation in 2017.PMID: 28196545 |
GTX109242 WB ImageThe data was published in the journal PLoS One in 2014.PMID: 24658465 |
GTX109242 IHC-P ImageThe data was published in the journal Oncotarget in 2016. PMID: 27637084 |
GTX109242 WB ImageThe data was published in the journal BMC Microbiol in 2017. PMID: 28835201 |
GTX109242 WB ImageThe data was published in the journal Sci Rep in 2017.PMID: 28211494 |
GTX109242 WB ImageThe data was published in the journal Oncotarget in 2016. PMID: 27637084 |
GTX109242 WB ImageThe data was published in the journal Physiol Rep in 2013. PMID: 24303100 |
Application Reference
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Arginase 1 antibody Cat. No. GTX109242
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