Carbonic anhydrase from bovine erythrocytes ca. 1.5 U/mg

Carbonic anhydrase preparation, which is homogeneous in SDS-PAGE. The enzyme preparation can therefore be used as a marker in protein gel electrophoresis and as a bioreagent in gel filtration chromatography, protein chromatography and plasma and blood proteins. Contains carbonic anhydrases A and B; both forms have similar high specific activities and therefore belong to the C-group of mammalian carbonic anhydrases (1).
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. It plays a main role in physiological processes like respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways.

Unit definition: 1 U catalyzes the hydrolysis of 1 µmole 4-nitrophenyl acetate per minute at 25 °C, pH 7.6 (2).
Activity in other units: ca. 4000 Wilbur-Anderson units/mg (3).


References:

1. Lindskog, S. et al. (1971) The Enzymes (Boyer, P.D., ed.) 5, 587-665
2. Armstrong, J. McD. et al. (1966) J. Biol. Chem. 241, 5137-49
3. Wilbur, K.M. & Anderson, N.G. (1948) J. Biol. Chem. 176, 147-54

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EINECS: 232-576-6 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C