Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

CCCP [555-60-2]

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Cat# NB-64-38873-100mg

Size : 100mg

Marca : Neo Biotech

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CCCP

(Synonyms: Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone) Copy Product Info

Synonyms: Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

Catalog No. T7081 Copy Product Info

Purity: 99.64%

CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.

Cas No. 555-60-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.64%

Color:White to Yellow

Product Introduction

Bioactivity

Description	CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.
Targets & IC50	HepG2 cells:6 μM, HepG2 cells:7.2 μM, HepG2 cells:9.8 μM
In vitro	METHODS: Human cervical cancer cells HeLa were treated with CCCP (20 μM) for 30 min, and the effect on the mitochondria-labeled fluorescent dye DiOC6 was observed using live cell fluorescence microscopy. RESULTS: CCCP-induced loss of mitochondrial membrane potential resulted in efflux of DiOC6 from mitochondria into the cytoplasm and subsequent localization to punctate structures. [1] METHODS: Mouse alveolar epithelial cells, MLE-12, were treated with CCCP (10-50 μM) for 2-18 h. The expression levels of the target proteins were examined by Western Blot. RESULTS: CCCP caused a dose- and time-dependent conversion of LC3-I to LC3-II. [2]
In vivo	METHODS: To assay in vivo activity, CCCP (5 mg/kg) was administered as a single intraperitoneal injection to adult and aged C57BL/6J mice, and hearts were collected 12 h later. RESULTS: Under CCCP-treated conditions, more mitochondria bound to autophagic vesicles were found in the hearts of adult but not aged mice. [3] METHODS: To assay in vivo activity, CCCP (1 mg/kg) was administered intraperitoneally to mt-Rosella mice once a day for three days. RESULTS: Mitochondrial fragments were readily visible on the hearts of CCCP-treated mice, and these fragments were trapped in lysosomes for degradation.CCCP induced mitochondrial autophagy. [4]
Synonyms	Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

Chemical Properties

Molecular Weight	204.62
Formula	C₉H₅ClN₄
Cas No.	555-60-2
Smiles	ClC1=CC=CC(N\N=C(/C#N)C#N)=C1
Relative Density.	1.3807 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (1233.99 mM), Sonication is recommended.

Ethanol: 5 mg/mL (24.44 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 6 mg/mL (29.32 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.8871 mL	24.4355 mL	48.8711 mL	244.3554 mL
5 mM	0.9774 mL	4.8871 mL	9.7742 mL	48.8711 mL
10 mM	0.4887 mL	2.4436 mL	4.8871 mL	24.4355 mL
20 mM	0.2444 mL	1.2218 mL	2.4436 mL	12.2178 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0977 mL	0.4887 mL	0.9774 mL	4.8871 mL
100 mM	0.0489 mL	0.2444 mL	0.4887 mL	2.4436 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

TMEM173TBK1STINGStimulator of Interferon GenesMPYSMitochondrialMetabolismMitochondrial MetabolismMITAIκBInterferon-α/β receptorInterferon-alpha/beta receptorInhibitorinhibitIKKIkB/IKKIkBIFN-βIFNARERISCCCPBacterialApoptosis

Related Tags: CCCP chemical structure | CCCP in vivo | CCCP in vitro | CCCP formula | CCCP molecular weight