Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (Biotin)

CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (Biotin)

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Cat# C2405-04V-Biotin-100ul

Size : 100ul

Marca : US Biological


C2405-04V-Biotin CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (Biotin)

Clone Type
Monoclonal
Host
rat
Source
human
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Ca Hu Mk Mo Po Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD49f can form distinct complexes with either the CD29-antigen (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Platelet VLA-6 functions as a laminin receptor.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): Reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts.
Optimal dilutions to be determined by the researcher.

IHC Postive Control:
Human Tonsil

Hybridoma:
Sp2/0 myeloma cells with spleen cells from immunized Sprague-Dawley rats.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H1515 (NKI-GoH3)|Host: rat|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: BALB/c mouse mammary tumor cells.|Specificity: Recognizes human CD49f. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). Reported to inhibit platelet adhesion to laminin. Species Crossreactivity: Mouse, canine, porcine, sheep, cynomolgus monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
BALB/c mouse mammary tumor cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD49f. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). Reported to inhibit platelet adhesion to laminin. Species Crossreactivity: Mouse, canine, porcine, sheep, cynomolgus monkey.
References
1. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W. et al. Oxford University Press p1029-1032, 2. Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055. Oxford University Press, 3. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. Histochem. Cytochem. 34: 1037-1046, 4. Sonnenberg, A. et al . (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody J. Biol. Chem. 262: 10376-10383, 5. Helmer, M. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures J. Biol. Chem. 263: 7660-7665, 6. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes. Vet. Immunol. Immunopathol. 53: 329 - 334, 7. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6. Nature. 336: 487 - 489, 8. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragments E8. J. Cell. Biol. 110: 2145 - 2155, 9. Sonnenberg, A., et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumour cells using a monoclonal antibody against platelet glycoprotein Ic. J. Biol. Chem. 263: 14030 - 14038, 10. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim.49 (2): 97-110, 11. Sonneberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J. Cell Sci. 96: 207 - 217.