Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> CD90 (Thy1.1) (FITC)

CD90 (Thy1.1) (FITC)

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Cat# C2441-04A-FITC-100ul

Size : 100ul

Marca : US Biological


C2441-04A-FITC CD90 (Thy1.1) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
rat
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IF IHC IP
Crossreactivity
Gp Mo Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilution determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-78Y: IgG1, Murine Negative Control (For rat tissue)

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 6A341 (OX-7)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rat Thy1 antigen. Swiss/UniProt Accession: P01830|Specificity: Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat Thy1 antigen. Swiss/UniProt Accession: P01830
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit
References
1. Mason, D.W. et al. (1980) Biochem.J. 187: 1-20. 2. Campbell, D.G. et al. (1981) Biochem. J. 195: 15-30. 3. Bukovský, A. et al. (1983) Immunology. 48:587-595. 4. Lee, W. et al. (1998) Circ. Res. 82: 845-851. 5. Jeng, C .J. et al. (1998) J. Cell. Biol. 140(3): 685-98. 6. Banerjee, S.A. et al. (1997) J. Neurosci. 17: 2756-2765.7. Kawachi, H. et al. (1992) Clin. Exp. Immunol. 88: 399-404.8. Tamura, M. et al. (1996) Biochem Biophys Res Comm.9. Stevenson, K.S. et al. (2009) Stem Cells Dev. 18:1389-98. 10. Biermann, J. et al. (2011) Mol Vis. 17: 395-403. 11. Keller, R.K. et al. (2004) J Lipid Res. 45: 347-5512. Ohashi, N. et al. (2010) Hypertens Res. 33:1174-81.