Applications

Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CnBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate esters or imidocarbonates. These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix. Lyophilized and stabilized with lactose.

Highlights:

• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Multi-point attachment of many protein ligands, resulting in a chemically stable product.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.

Available in bulk (inquire) as lyophilized matrix.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

• 1g swell to ~ 5 ml

References

Novel extraction supports based on immobilised aptamers: Evaluation for the selective extraction of cocaine. Talanta. (2011) 85: 616-24.
New approach to quantitative analysis of benzopmpyrene in food supplements by an immunochemical column test. Talanta. (2011) 85: 151-6.
Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum. J Parasit Dis. (2010) 34: 57-61.
Protein hydrolysis by immobilized and stabilized trypsin. Biotechnol Prog. (2011) 27: 677-83.
Preparation and characterization of an immunoaffinity column for the selective extraction of salbutamol from pork sample. J Chromatogr Sci. (2011) 49: 276-80.
A proteome-scale study on in vivo protein Nα-acetylation using an optimized method. Proteomics. (2011) 11: 81-93.
Molecular cloning and biochemical characterization of a myotoxin inhibitor from Bothrops alternatus snake plasma. Biochimie. (2011) 93: 583-92.
Establishment of an immunoaffinity chromatography for simultaneously selective extraction of Sudan I, II, III and IV from food samples. J Chromatogr A. (2010) 1217: 7840-7.
Quantitative detection of trace systemins in Solanaceous plants by immunoaffinity purification combined with liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry. Anal Chem. (2010) 82: 9374-83.
Epigallocatechin-3-gallate suppresses the expression of HSP70 and HSP90 and exhibits anti-tumor activity in vitro and in vivo. BMC Cancer. (2010) 10: 276.
Determination of epitestosterone in human urine by off-line immunoaffinity solid-phase extraction coupled with high performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. (2010) 878: 1443-8.
One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides. Acta Biochim Pol. (2010) 57: 89-93.
Preparation and characterization of an immunoaffinity chromatography column for the selective extraction of trace contraceptive drug levonorgestrel from water samples. Talanta. (2009) 80: 98-103.
A proteomics approach to study in vivo protein N(alpha)-modifications. J Proteomics. (2009) 73: 240-51.
Purification process development for HER1 extracellular domain as a potential therapeutic vaccine. J Chromatogr B Analyt Technol Biomed Life Sci. (2009) 877: 3105-10.
Preparation of acetochlor antibody and its application on immunoaffinity chromatography cleanup for residue determination in peanuts. J Agric Food Chem. (2009) 57: 7640-3.
Determination of cocaine in human plasma by selective solid-phase extraction using an aptamer-based sorbent. Anal Chem. (2009) 81: 7081-6.
Flow injection analysis of angiotensin I-converting enzyme inhibitory activity with enzymatic reactors. Talanta. (2009) 79: 1130-4.
Immunoglobulin G-reactive antibodies from sera of healthy individuals enriched in IgG2--therapeutic potential in HIV-1 infection. Curr HIV Res. (2009) 7: 378-83.
Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal antibodies. J AOAC Int. (2008) 91: 1488-93.
Determination of microcystin-LR in water from Lake Tai, China. Bull Environ Contam Toxicol. (2009) 82: 230-3.
Evaluation of various immobilized enzymatic microreactors coupled on-line with liquid chromatography and mass spectrometry detection for q