Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EEA1 antibody [C3], C-term (GTX109638) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg A375 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Sci Rep in 2016.PMID: 26838546

The data was published in the journal Virol J in 2012. PMID: 22339884

The data was published in the journal Virol J in 2012. PMID: 22339884