F4/80 antibody [BM8.1] (APC-Cy7)
Cat# GTX01470-15-100ug
Size : 100ug
Marca : Genetex
Host | Rat |
---|---|
Clonality | Monoclonal |
Clone Name | BM8.1 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Mouse |
Package | 100 μg |
View product citations for antibody GTX01470-15 on CiteAb
PRODUCT
Summary
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application | Recommended Dilution |
---|---|
FACS | Assay dependent |
Not tested in other applications.
Calculated MW
102 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Allophycocyanin-Cyanine7 (APC-Cy7)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Cellular Localization
Cell membrane
Database
DATA IMAGES
GTX01470-15 FACS Image FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-15 F4/80 antibody [BM8.1] (APC-Cy7). |
Clone Reference
2013; Inflammatory monocytes are critical for induction of a polysaccharide-specific antibody response to an intact bacterium.
2013; Macrophage-specific TLR2 signaling mediates pathogen-induced TNF-dependent inflammatory oral bone loss.
2013; In vivo ablation of plasmacytoid dendritic cells inhibits autoimmunity through expansion of myeloid-derived suppressor cells.
2010; IL-33 induces IL-13-dependent cutaneous fibrosis.
2005; Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
REVIEW
Clone Reference
2013; Inflammatory monocytes are critical for induction of a polysaccharide-specific antibody response to an intact bacterium.
2013; Macrophage-specific TLR2 signaling mediates pathogen-induced TNF-dependent inflammatory oral bone loss.
2013; In vivo ablation of plasmacytoid dendritic cells inhibits autoimmunity through expansion of myeloid-derived suppressor cells.
2010; IL-33 induces IL-13-dependent cutaneous fibrosis.
2005; Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
SDS | |
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Sodium Azide.pdf |