Griffonia simplicifolia GS I (Bandeiraea simplicifolia)

Cat# G8967-12-5mg

Size : 5mg

Marca : US Biological

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G8967-12 Griffonia simplicifolia GS I (Bandeiraea simplicifolia)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Applications
E
Shipping Temp
Blue Ice
Storage Temp
-20°C

GSL I is a family of glycoproteins with molecular weights of approximately 115,000. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for a-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues. Our GSL I is a mixture of the five isolectins. Binding appears to require divalent cations such as calcium and magnesium. GSL I has been reported to bind several glycoproteins including laminin.||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Inhibiting/Eluting Sugar: |200mM galactose/200mM N-acetylgalactosamine mixture||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 10mM HEPES, pH 8.5, 0.1mM Ca++. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Griffonia (Bandeiraea) simplicifolia seeds|Purity: Homogeneous by SDS-PAGE|Concentration: Not Determined |Form: Supplied as a lyophilized powder. Reconstitute with 10mM HEPES, pH 8.5, 0.1mM Ca++. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder. Reconstitute with 10mM HEPES, pH 8.5, 0.1mM Ca++.
Purity
Homogeneous by SDS-PAGE