HIV-1 Protease Recombinant
HIV-1 Protease Recombinant
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Product Description
Specifications
| Size | 1000 µg |
| Expressed Region | PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF |
| Expression System | E. coli |
| Purity | Greater than 95% as determined by densitometric image analysis. |
| Format | Sterile filtered colorless clear solution. |
| Formulation | The HIV-1 Protease filtered (0.4um) solution (0.386mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5. |
| Research Area | Infectious Disease, HIV |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).
Kinetic parameters
Km=15.1 uM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) .. p-nitrophenylalanine).
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.