Human Angiogenesis Array Q3

Cat# QAH-ANG-3-1

Size : 8SampleKit

Marca : RayBiotech


Human Angiogenesis Array Q3

Quantibody® Human Angiogenesis Array 3 Kit. Detects 30 Human Angiogenic Factors. Suitable for all liquid sample types.

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected30
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaAngiogenesis, Vascular Biology
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Angiopoietin-1
  • Gene Symbol: ANGPT1
  • Gene ID: 284
  • Accession #: Q15389
  • ELISA Kit: RayBio Human ANGPT1 ELISA
Angiostatin
  • Gene Symbol: PLG
  • Gene ID: 5340
  • Accession #: P00747
  • ELISA Kit: RayBio Human Angiostatin ELISA
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: 58191
  • Accession #: Q9H2A7
  • ELISA Kit: RayBio Human CXCL16 ELISA
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
  • ELISA Kit: RayBio Human EGF ELISA
FGF-4
  • Gene Symbol: FGF4
  • Gene ID: 2249
  • Accession #: P08620
  • ELISA Kit: RayBio Human FGF-4 ELISA
Follistatin
  • Gene Symbol: FST
  • Gene ID: 10468
  • Accession #: P19883
  • ELISA Kit: RayBio Human Follistatin ELISA
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
  • ELISA Kit: RayBio Human G-CSF ELISA
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
  • ELISA Kit: RayBio Human GM-CSF ELISA
I-309
(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
  • ELISA Kit: RayBio Human I-309 ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
  • ELISA Kit: RayBio Human IL-1 beta ELISA
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
  • ELISA Kit: RayBio Human IL-4 ELISA
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
  • ELISA Kit: RayBio Human IL-10 ELISA
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
  • ELISA Kit: RayBio Human IL-12 p40 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
  • ELISA Kit: RayBio Human IL-12 p70 ELISA
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: 6373
  • Accession #: O14625
  • ELISA Kit: RayBio Human I-TAC ELISA
MCP-2
(CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
  • ELISA Kit: RayBio Human MCP-2 ELISA
MCP-3
(MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
  • ELISA Kit: RayBio Human MCP-3 ELISA
MCP-4
(CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
  • ELISA Kit: RayBio Human MCP-4 ELISA
MMP-1
  • Gene Symbol: MMP1
  • Gene ID: 4312
  • Accession #: P03956
  • ELISA Kit: RayBio Human MMP-1 ELISA
MMP-9
  • Gene Symbol: MMP9
  • Gene ID: 4318
  • Accession #: P14780
  • ELISA Kit: RayBio Human MMP-9 ELISA
PECAM-1
(CD31)
  • Gene Symbol: PECAM1
  • Gene ID: 5175
  • Accession #: P16284
  • ELISA Kit: RayBio Human PECAM-1 ELISA
TGF alpha
  • Gene Symbol: TGFA
  • Gene ID: 7039
  • Accession #: P01135
  • ELISA Kit: RayBio Human TGF-alpha ELISA
TGF beta 3
 
Tie-1
  • Gene Symbol: TIE1
  • Gene ID: 7075
  • Accession #: P35590
  • ELISA Kit: RayBio Human Tie-1 ELISA
Tie-2
  • Gene Symbol: TEK
  • Gene ID: 7010
  • Accession #: Q02763
  • ELISA Kit: RayBio Human Tie-2 ELISA
uPAR
  • Gene Symbol: PLAUR
  • Gene ID: 5329
  • Accession #: Q03405
  • ELISA Kit: RayBio Human uPAR ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
  • ELISA Kit: RayBio Human VEGF-A ELISA
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: 3791
  • Accession #: P35968
  • ELISA Kit: RayBio Human VEGF R2 ELISA
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: 2324
  • Accession #: P35916
  • ELISA Kit: RayBio Human VEGF R3 ELISA
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: 2277
  • Accession #: O43915
  • ELISA Kit: RayBio Human VEGF-D ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Angiogenesis Array Q3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAH-ANG-3.gal)
Excel (QAH-ANG-3_Analysis_Tool.xls)

citations

  • Serum differentially expressed angiogenic cytokines in head and neck vascular malformations

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Journal of Oral Pathology & Medicine
  • Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy

    Species:
    Human
    Sample Type:
    Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)
    Year:
    2019
    PubMed ID:
    30225999
  • (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells

    Species:
    Human
    Sample Type:
    Cell Lysate (Microvesicles and MSC parents)
    Year:
    2015
    Journal Name:
    Plos One
    PubMed ID:
    25514634
  • Carbamylated low-density lipoprotein induces oxidative stress and accelerated senescence in human endothelial progenitor cells

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2011
    Journal Name:
    The FASEB Journal

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