CSB-E08160h-96T | Human Gelsolin ELISA Kit Clinisciences

Human Gelsolin ELISA Kit

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Product Details

Citations

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Marca : Cusabio

Cat# CSB-E08160h-96T

Associated names

Actin depolymerizing factor ELISA Kit; Actin-depolymerizing factor ELISA Kit; ADF ELISA Kit; AGEL ELISA Kit; Brevin ELISA Kit; DKFZp313L0718 ELISA Kit; GELS_HUMAN ELISA Kit; Gelsolin ELISA Kit; Gsn ELISA Kit

Abbreviation

Uniprot No.

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2.74 ng/ml - 2000 ng/ml

Sensitivity

2.74 ng/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human gelsolin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-103
1:2	Average %	94
	Range %	88-100
1:4	Average %	90
	Range %	86-95
1:8	Average %	91
	Range %	85-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2000

2.735

2.765

2.750

2.665

666.66

1.995

2.130

2.063

1.978

222.22

1.358

1.334

1.346

1.261

74.07

0.747

0.751

0.749

0.664

24.69

0.461

0.435

0.448

0.363

8.23

0.232

0.223

0.228

0.143

2.74

0.191

0.195

0.193

0.108

0

0.086

0.084

0.085

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GSN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GSN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

The Human Gelsolin (GSN) ELISA Kit quantifies GSN in human serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for metabolic research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which GSN in the samples or standards are sandwiched between pre-coated GSN a...

The recovery of human gelsolin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

EDTA plasma (n=4)