Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Hydrocortisone [50-23-7]

Stampa

Cat# HY-N0583-1g

Size : 1g

Marca : MedChemExpress

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Description

Hydrocortisone (Cortisol) is a steroid hormone or glucocorticoid secreted by the adrenal cortex^[1].

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 26695732]
B16-F10	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 26695732]
COS-7	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against african green monkey COS1 cells after 72 hrs by MTT assay Cytotoxicity against african green monkey COS1 cells after 72 hrs by MTT assay	[PMID: 26695732]
HeLa	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 26695732]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Hydrocortisone	TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells	[PMID: 12699389]
Macrophage	IC₅₀	10 nM Compound: Hydrocortisone	Antiinflammatory activity in CD-1 mouse Macrophage assessed as inhibition of IFN-gamma induced NO production after 48 hrs by Griess reaction Antiinflammatory activity in CD-1 mouse Macrophage assessed as inhibition of IFN-gamma induced NO production after 48 hrs by Griess reaction	[PMID: 21361338]
MCF7	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 26695732]
NIH3T3	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay	[PMID: 26695732]
RAW264.7	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against mouse RAW264.7 cells after 72 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells after 72 hrs by MTT assay	[PMID: 26695732]
RAW264.7	IC₅₀	3.26 μM Compound: Hydrocortisone	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production in macrophages Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production in macrophages	[PMID: 32485533]
RAW264.7	IC₅₀	3.4 μM Compound: Hydrocortisone	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay	[PMID: 33454546]
RAW264.7	IC₅₀	40.64 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay	[PMID: 20879743]
RAW264.7	IC₅₀	40.64 μM Compound: Hydrocortisone	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry	[PMID: 21807513]
RAW264.7	IC₅₀	40.64 μM Compound: Hydrocortisone	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction	[PMID: 23067550]
RAW264.7	IC₅₀	43.8 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23755850]
RAW264.7	IC₅₀	5.5 μM Compound: Hydrocortisone	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs	[PMID: 31126853]
RAW264.7	IC₅₀	51.4 μM Compound: hydrocortisone	Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess assay	[PMID: 25275213]
RAW264.7	IC₅₀	52.43 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS addition measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS addition measured after 24 hrs by Griess assay	[PMID: 31009914]
RAW264.7	IC₅₀	53.7 μM Compound: hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of NO production after 24 hrs by Griess assay	[PMID: 25076059]
RAW264.7	IC₅₀	53.8 μM Compound: Hydrocortisone	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells treated 1 hr before LPS challenge measured after 24 hrs by Griess reaction method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells treated 1 hr before LPS challenge measured after 24 hrs by Griess reaction method	[PMID: 23294419]
RAW264.7	IC₅₀	54.23 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 24660966]
RAW264.7	IC₅₀	54.23 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 26615888]
RAW264.7	IC₅₀	58.79 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 27256914]
RAW264.7	IC₅₀	58.79 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 27295506]
RAW264.7	IC₅₀	58.79 μM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS addition and measured after 24 hrs by griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS addition and measured after 24 hrs by griess assay	[PMID: 30419492]
RAW264.7	IC₅₀	61 nM Compound: Hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction	[PMID: 21361338]
RAW264.7	IC₅₀	63.86 μM Compound: hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production by ELISA	[PMID: 20095629]
RAW264.7	IC₅₀	64.34 μM Compound: hydrocortisone	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition LPS-induced nitric oxide production cells after 24 hrs Antiinflammatory activity in mouse RAW264.7 assessed as inhibition LPS-induced nitric oxide production cells after 24 hrs	[PMID: 20095629]
RAW264.7	IC₅₀	64.34 μM Compound: Hydrocortisone	Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method	[PMID: 23305920]
RAW264.7	IC₅₀	64.34 μM Compound: hydrocortisone	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation	[PMID: 18348534]
RAW264.7	IC₅₀	85.64 μM Compound: hydrocortisone	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production by ELISA	[PMID: 20095629]
SK-OV-3	IC₅₀	>20 μM Compound: HYD	Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay	[PMID: 26695732]

In Vitro

Hydrocortisone (50 nM) shows a dose-dependent down-regulation of GR transcript in hCMEC/D3 cells. Hydrocortisone supplementation of the serum-reduced cell differentiation medium leads to a significant increase in TER across the hCMEC/D3 monolayer^[1]. Hydrocortisone-treated Dendritic cells (DCs) show a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibit production of IFN-γ but induce an increased release of IL-4 and no change in IL-5^[2]. Hydrocortisone reduces postischemic oxidative stress, perfusion pressure, and transudate formation. Hydrocortisone inhibits postischemic shedding of syndecan-1, heparan sulfate, and hyaluronan as is release of histamine from resident mast cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvant et solubilité

In Vitro:

DMSO : 200 mg/mL (551.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 5 mg/mL (13.79 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7589 mL	13.7946 mL	27.5893 mL
5 mM	0.5518 mL	2.7589 mL	5.5179 mL
10 mM	0.2759 mL	1.3795 mL	2.7589 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Pureté et documentation

Purity: 99.92%

Fiche technique (279 KB) SDS (419 KB)

COA (252 KB) HNMR (141 KB) RP-HPLC (255 KB) MS (68 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Förster C, et al. Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol. 2008 Apr 1;586(7):1937-49. [Content Brief]

[2]. Bellinghausen I, et al. Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: differences from hydrocortisone-treated dendritic cells. J Allergy Clin Immunol. 2001 Aug;108(2):242-9. [Content Brief]

[3]. Chappell D, et al. Hydrocortisone preserves the vascular barrier by protecting the endothelial glycocalyx. Anesthesiology. 2007 Nov;107(5):776-84. [Content Brief]

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Hydrocortisone [50-23-7]

Cat# HY-N0583-1g

Marca : MedChemExpress

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