Insulin Like Growth Factor 1 (IGF1) Recombinant, Mouse

Source:
Recombinant Mouse from E. coli

Purity:
≥97%

Endotoxin:
1.0EU per 1ug (determined by the LAL method)

Accession No:
Q8CAR0

Fragment:
Gly33~Ala102 (Accession No: Q8CAR0)

Sequence:
MGHHHHHHSGSEF-GPETLCGA ELVDALQFVC GPRGFYFNKP TGYGSSIRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPTK AA

Epitope Tag:
N-terminal His-tag

Molecular Weight:
9.2kD

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.