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> Isomaltotetraose [35997-20-7]

Isomaltotetraose [35997-20-7]

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Cat# HY-N7948-1mL

Size : 10mM/1mL

Marca : MedChemExpress


Description

Isomaltotetraose is an orally effective branched isomaltooligosaccharide with α-(1,6) glycosidic linkages. Isomaltotetraose is a prebiotic oligosaccharide present in honey and also a component of isomaltooligosaccharide mixtures. Isomaltotetraose maintains the growth of *Prevotella* or sustains the *Prevotella* enterotype in both in vitro systems and mouse models[1][2][3].

In Vitro

Isomaltotetraose can be produced in an IMO mixture with 25.0% isomaltotetraose within 20 h of synchronous incubation through the synergistic action of Bacillus stearothermophilus
Maltogenic amylase (HY-E70325) (BSMA) and α-GTase on liquefied corn starch; in contrast, sequential addition of α-GTase without inactivating BSMA reduces the isomaltotetraose content, thus favoring the production of longer-chain IMO[1].
The Prevotella enterotype is one of the three major structural types of the human gut microbiota, characterized by the dominance of Prevotella bacteria in the gut. This type of microbiota is highly adept at degrading plant-derived complex polysaccharides and contributes to weight management and metabolic health[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isomaltotetraose Related Antibodies

Solvant et solubilité
In Vitro: 

H2O : 100 mg/mL (150.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5002 mL 7.5010 mL 15.0019 mL
5 mM 0.3000 mL 1.5002 mL 3.0004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (75.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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