Application
| WB, IHC, IF, E |
|---|---|
| Primary Accession | Q9UGL1 |
| Other Accession | NM_006618 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 1G10 |
| Calculated MW | 175658 Da |
| Gene ID | 10765 |
|---|---|
| Other Names | Lysine-specific demethylase 5B, 11411-, Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1, KDM5B, JARID1B, PLU1, RBBP2H1 |
| Target/Specificity | KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | JARID1B Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
References
1.Jumonji/ARID1 B (JARID1B) Protein Promotes Breast Tumor Cell Cycle Progression through Epigenetic Repression of MicroRNA let-7e.Mitra D, Das PM, Huynh FC, Jones FE.J Biol Chem. 2011 Nov 25;286(47):40531-5. Epub 2011 Oct 3.2.Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway.Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BA, Nakamura Y, Hamamoto R.Mol Cancer. 2010 Mar 13;9:59.3.JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.Xiang Y, Zhu Z, Han G, Ye X, Xu B, Peng Z, Ma Y, Yu Y, Lin H, Chen AP, Chen CD.Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19226-31. Epub 2007 Nov 28.