LEP ELISA Kit (Chicken)
Cat# OKEH03954
Size : 96Wells
Marca : Aviva Systems Biology
LEP ELISA Kit (Chicken) (OKEH03954)
| Datasheets/Manuals | Printable datasheet for LEP ELISA Kit (Chicken) (OKEH03954) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology LEP ELISA Kit (Chicken) (OKEH03954) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEP captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.089 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Leptin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | LEP |
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| Gene Full Name | leptin |
| Alias Symbols | Obesity factor. |
| NCBI Gene Id | 373955 |
| Protein Name | Leptin |
| Description of Target | Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. |
| Uniprot ID | O42164 |
| Protein Size (# AA) | 163 |
| Molecular Weight | 163 kDa |
