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> Mouse Cytokine Array C4

Mouse Cytokine Array C4

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Cat# AAM-CYT-4-2

Size : 2SampleKit

Marca : RayBiotech

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Mouse Cytokine Array C4

RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size 2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species Mouse
Quantitative/Semi-Quantitative Semi-Quantitative
Number of Targets Detected 34
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support Membrane
Method Of Detection Chemiluminescence
Design Principle Sandwich-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF
  • Gene Symbol: FGF2
  • Accession #:
  • ELISA Kit:
CD26
(DPPIV)
  • Gene Symbol: DPP4
  • Accession #:
  • ELISA Kit:
Dtk
  • Gene Symbol: TYRO3
  • Accession #:
  • ELISA Kit:
E-Selectin
  • Gene Symbol: SELE
  • Accession #:
  • ELISA Kit:
Fc gamma RIIB
(CD32b)
  • Gene Symbol: FCGR2B
  • Accession #:
  • ELISA Kit:
Flt-3 Ligand
  • Gene Symbol: FLT3LG
  • Accession #:
  • ELISA Kit:
GITR
(TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Accession #:
  • ELISA Kit:
HGFR
  • Gene Symbol: MET
  • Accession #:
  • ELISA Kit:
ICAM-1
(CD54)
  • Gene Symbol: ICAM1
  • Accession #:
  • ELISA Kit:
IGFBP-2
  • Gene Symbol: IGFBP2
  • Accession #:
  • ELISA Kit:
IGF-1
  • Gene Symbol: IGF1
  • Accession #:
  • ELISA Kit:
IGF-2
  • Gene Symbol: Igf2
  • Accession #:
  • ELISA Kit:
IL-15
  • Gene Symbol: IL15
  • Accession #:
  • ELISA Kit:
IL-17 RB
  • Gene Symbol: IL17RB
  • Accession #:
  • ELISA Kit:
IL-7
  • Gene Symbol: IL7
  • Accession #:
  • ELISA Kit:
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Accession #:
  • ELISA Kit:
Lungkine
(CXCL15)
  • Gene Symbol: CXCL15
  • Accession #:
  • ELISA Kit:
MDC
(CCL22)
  • Gene Symbol: CCL22
  • Accession #:
  • ELISA Kit:
MMP-2
  • Gene Symbol: MMP2
  • Accession #:
  • ELISA Kit:
MMP-3
  • Gene Symbol: MMP3
  • Accession #:
  • ELISA Kit:
Osteopontin
(SPP1)
  • Gene Symbol: SPP1
  • Accession #:
  • ELISA Kit:
Osteoprotegerin
(TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Accession #:
  • ELISA Kit:
Pro-MMP-9
  • Gene Symbol: Mmp9
  • Accession #:
  • ELISA Kit:
Resistin
  • Gene Symbol: RETN
  • Accession #:
  • ELISA Kit:
Sonic Hedgehog N-Terminal
(Shh-N)
  • Gene Symbol: SHH
  • ELISA Kit:
TCK-1
(CXCL7)
  • Gene Symbol: Ppbp
  • Accession #:
  • ELISA Kit:
TIMP-2
  • Gene Symbol: TIMP2
  • Accession #:
  • ELISA Kit:
TRANCE
(TNFSF11)
  • Gene Symbol: TNFSF11
  • Accession #:
  • ELISA Kit:
TROY
(TNFRSF19)
  • Gene Symbol: TNFRSF19
  • Accession #:
  • ELISA Kit:
TSLP
  • Gene Symbol: TSLP
  • Accession #:
  • ELISA Kit:
VEGFR1
  • Gene Symbol: Flt1
  • Accession #:
  • ELISA Kit:
VEGFR2
  • Gene Symbol: KDR
  • Accession #:
  • ELISA Kit:
VEGFR3
  • Gene Symbol: FLT4
  • Accession #:
  • ELISA Kit:
VEGF-D
  • Gene Symbol: FIGF
  • Accession #:

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.