PAMP-12 (unmodified) is an endogenous peptide and is a MrgX2 agonist. PAMP-12 (unmodified) can reduce cAMP accumulation, increase Ca²⁺ levels, enhance beta-arrestin recruitment, decrease IP-1, and increases phosphoERK. PAMP-12 (unmodified) can elicit hypotension through inhibiting catecholamine secretion from sympathetic nerve endings and adrenal chromaffin cells. PAMP-12 (unmodified) can be used for the research of hypotension and ulcerative colitis^[1][2].

PAMP-12 (10^-9-10^-6 M; 30 min) potently inhibits Forskolin (HY-15371)-elevated cAMP accumulation in CHO-MrgX2 cells with an EC₅₀ of 57.2 nM^[1].
PAMP-12 (10^-10-10^-6 M) potently induces intracellular Ca²⁺ mobilization in CHO-MrgX2 cells with an EC₅₀ of 41.0 nM^[1].
PAMP-12 (10^-10-10^-6 M; 60 min) directly binds to and activates MrgX2 in HEK293 cell membranes, stimulating [³⁵S]GTPγS binding with an EC₅₀ of 20.8 nM^[1].
PAMP-12 (90 min) stimulation induces greater β-arrestin recruitment to the MRGPRX2 62Ser variant^[2].
PAMP-12 (10^-7-10^-4 M; 2 h) stimulation results in greater IP-1 accumulation, indicating stronger Gq/phospholipase C pathway activation, in CHO cells expressing the MRGPRX2 Asn62 variant compared to the 62Ser variant^[2].
PAMP-12 (10-20 μM; 0.5-5 min) stimulation induces greater pERK activation in HMC-1.1 cells expressing the MRGPRX2^62Ser variant compared to the Asn62 variant^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1619.91

C₇₇H₁₁₈N₂₄O₁₅

929905-12-4

Solid

White to off-white

Phe-Arg-Lys-Lys-Trp-Asn-Lys-Trp-Ala-Leu-Ser-Arg

FRKKWNKWALSR

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Kamohara M, et al. Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides[J]. Biochemical and biophysical research communications, 2005, 330(4): 1146-1152.

  [2]. Chen E, et al. Inflamed Ulcerative Colitis Regions Associated With MRGPRX2-Mediated Mast Cell Degranulation and Cell Activation Modules, Defining a New Therapeutic Target. Gastroenterology. 2021;160(5):1709-1724.  [Content Brief]