PD-L1 antibody
Cat# GTX104763-25ul
Size : 25ul
Marca : Genetex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS |
Reactivity | Human |
Package | 100 μl, 25 μl |
Summary
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | Assay dependent |
FACS | Assay dependent |
Calculated MW
Observed MW
Positive Control
Product Note
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX104763 WB ImageWild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX104763 WB Image Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:1200 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX104763 ICC/IF ImagePD-L1 antibody detects PD-L1 protein at cell membrane by immunofluorescent analysis.Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920). |
GTX104763 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
GTX104763 WB ImageNon-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX104763 WB ImageVarious whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and with DDDDK tag antibody (GTX115043) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX104763 IHC-P ImagePD-L1 antibody detects PD-L1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX104763 ICC/IF ImagePD-L1 antibody detects PD-L1 protein by immunofluorescent analysis.Sample: MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm. |
GTX104763 IHC-P Image PD-L1 antibody detects PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. |
GTX104763 WB ImageUntreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. |
GTX104763 WB Image Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:2000 and competitor's antibody (CST#13684) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX104763 IHC-P Image PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology. |
GTX104763 IHC-P Image PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. |
GTX104763 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
GTX104763 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31905966 |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31835799 |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31835799 |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31905966 |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31835799 |
GTX104763 WB ImageThe data was published in the 2021 in Cancer Metab. PMID: 33608051 |
GTX104763 WB ImageThe data was published in the journal Nat Commun in 2020. PMID: 32245950 |
GTX104763 WB ImageThe data was published in the journal Nat Commun in 2020. PMID: 32245950 |
GTX104763 WB ImageThe data was published in the journal Cancers (Basel) in 2019.PMID: 31835799 |
Application Reference
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PD-L1 antibody Cat. No. GTX104763
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PBS.pdf | |
Glycerol.pdf | |
Proclin.pdf |