RayBio® Rat BDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Rat BDNF ELISA Kit
Rat BDNF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Rat |
| Sensitivity | 12 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 12 pg/ml - 3,000 pg/ml |
| Specificity | This ELISA antibody pair detects rat and human BDNF. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10 fold |
| Accession Number | P23363 |
| Gene Id | 24225 |
| Gene Symbols | BDNF |
| Protein Name / Synonyms | Brain-derived neurotrophic factor (BDNF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Rat BDNF qIPCR ELISA Kit
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat BDNF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified BDNF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Dysregulation of protein degradation and alteration of secretome in α-synuclein-exposed astrocytes: implications for dopaminergic neuronal dysfunction
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (conditioned media of astrocytes or DA neurons)
- Year:
- 2024
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 39617881
Callosal glia, GABAergic interneurons, and apoptotic profiles in the brains of cypermethrin-exposed male and female rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (callosal tissue homogenate)
- Year:
- 2026
- Journal Name:
- BMC Research Notes
- PubMed ID:
- 42216073
Neuroprotective potential of a plant-based intervention in diabetic neuropathy: Biochemical and behavioral insights from a streptozotocin-induced rat model
- Species:
- Rat
- Sample Type:
- Serum; Tissue Lysate / Homogenate (sciatic nerve homogenates)
- Year:
- 2026
- Journal Name:
- World journal of diabetes
- PubMed ID:
- 41696095
Modulation of TNFα-driven neuroinflammation by Gardenin A: insights from in vitro, in vivo, and in silico studies
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain tissue homogenates)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 41293241
Influence of ApoE genotype on doxorubicin-induced cognitive impairment in juvenile rats
- Species:
- Rat
- Sample Type:
- Serum; Tissue Lysate / Homogenate (cortex or hippocampus lysates)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 41436539
The potential of esculin in ameliorating Type-2 diabetes mellitus induced neuropathy in Wistar rats and probing its inhibitory mechanism of insulin aggregation
- Species:
- Rat
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- International Journal of Biological Macromolecules
CROCIN'S PROTECTIVE EFFECT AGAINST A VALPROATE-EXPOSED MODEL OF AUTISM SPECTRUM DISORDER: A HISTOLOGICAL AND OXIDO-INFLAMMATORY INVESTIGATION
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain)
- Year:
- 2025
- Journal Name:
- Journal of Applied Pharmaceutical Sciences and Research
Intranasal Delivery of a Silymarin Loaded Microemulsion for the Effective Treatment of Parkinson’s Disease in Rats: Formulation, Optimization, Characterization, and In Vivo Evaluation
- Species:
- Rat
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- Pharmaceutics
Microglial depletion and repopulation in brain slice culture normalizes sensitized proinflammatory signaling
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (OHSC culture media)
- Year:
- 2020
- Journal Name:
- Journal of Neuroinflammation
- PubMed ID:
- 31954398
Bioprinted Schwann and Mesenchymal Stem Cell Co-Cultures for Enhanced Spatial Control of Neurite Outgrowth
- Species:
- Rat
- Sample Type:
- mesenchymal stem cells (MSCs), Schwann cells (SC)
- Year:
- 2023
- Journal Name:
- Gels (Basel, Switzerland)
