- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IL-13 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-13 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
Rat Interleukin 13,IL-13 ELISA KIT
Cat# CSB-E07454r-96T
Size : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
interleukin 13
Associated names
Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-103 | |||||
Recovery
| The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 91-96 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat IL13 ELISA Kit was designed for the quantitative measurement of Rat IL13 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
Citations
- KM Moftah, WG Hozayen, NA Hasona,Stem Cells and Development,2026
- C Chen, H Han, H Li, Z Zhang, Y Lv,Neuroscience Letters,2025
- JS Park, YS Lee, JW Seo, HJ Na,Laboratory Animal Research,2025
- NM Atia, YM Shahine, OY Abdallah, MSA Ghany,AAPS PharmSciTech,2025
- Y Xia, P Bin, Y Zhou, M Zhao, J Zhang, W Zhong,Cell Reports,2025
- JH Oh, SH Baek, HW Seo, SH Lee,Journal of Ginseng Research,2025
- A Chen,Advanced healthcare materials,2023
- H Liu,Cell transplantation,2023
- Y Chen,Journal of Orthopaedic Translation,2022
- AA Shokry,Biomedicine & Pharmacotherapy,2021
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
Gene References into Functions
- SUMF2 may mediate airway inflammation in allergic asthma by modulating the expression of IL-13
- IL-13 significantly enhanced C/EBP-alpha/COX-2 expression and PGE2 production in LPS-treated microglial cells. Paradoxically, IL-13 abolished C/EBP-beta/PPAR-gamma/HO-1 expression.
- asthma the ERK expression and activation levels were increased, as was the protein level of IL-13. The ERK signaling pathway may be involved in the increased expression of the Th2 cytokine IL-13 in asthma.
- The IL-13 system is a novel regulator of paSMC growth. Dysregulation of IL-13 receptor expression in IPAH may partially underlie smooth muscle hypertrophy associated with pathological vascular remodeling in IPAH.
- IL-13 is involved in tobacco smoke-induced changes in the structure and function of rat intrapulmonary airways
- This finding provides an unequivocal evidence of IL-13 participation in pancreatic fibrosis, illustrating a new strategy for chronic pancreatitis.
- Inhibition of HO-1 with tin protoporphyrin reversed cytoprotective/antiapoptotic effects of IL-13 in liver transplantation.
- IL-13 may control brain inflammation by inducing the death of activated microglia in vivo, resulting in an enhancement of neuronal survival.
- study demonstrated at the protein and mRNA levels, both in vivo and in vitro, that alveolar macrophages produce IL-25 and IL-13 in response to fine particles of TiO2
- IL-13 overexpression in the rat could lead to podocyte injury and minimal-change-like nephropathy.
- The findings support a role for IL-13 as an in vivo antiangiogenic factor and provide a rationale for its use in RA to control. pathologic neovascularization.
- IL-13 down-regulates the cytokine-induced iNOS transcription by decreasing iNOS specific IRF-1/ISRE binding activity
- expression decreased in lung in response to alcohol exposure
- Endogenous IL-13 triggers microglial NADPH oxidase-derived oxidative stress, leading to degeneration of hippocampal neurons in vivo.
Subcellular Location
Secreted.
Protein Families
IL-4/IL-13 family
Database Links
KEGG:
STRING:
UniGene: