Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

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Cat# orb1947959-48T

Size : 48T

Marca : Biorbyt


Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

SKU: orb1947959

Description

Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

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Application Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TPH1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TPH1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TPH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TPH1. You can calculate the concentration of Rat TPH1 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityRat
Sample TypesTissue homogenate and Cell lysate
Assay TypeSandwich
Assay Time3.5H
Range0.32-20ng/mL
Sensitivity0.19 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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TPH1
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Quick Database Links

Gene Symbol
TPH1
UniProt
P09810

UniProt Details

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No UniProt data available

Protocol Information