Orb780600-48Tests | Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

SKU: orb780600

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Description

The Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Tryptophan Hydroxylase 2 (TPH2) in rat tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.57 ng/mL.

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPH2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPH2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.57 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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NTPH ELISA Kit, Neuronal tryptophan hydroxylase ELISA Kit, Tryptophan 5-monooxygenase 2 ELISA Kit

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Rat TPH2 ELISA Kit [orb411117]
Reactivity:
Rat
Dynamic Range:
31.25 pg/mL-2000 pg/mL
Sensitivity:
7.8 pg/mL
Sizes Available: 48 T, 96 T, 5 x 96 T, 10 x 96 T, 24 T
Rat TPH2 ELISA Kit (Ready to Use) [orb555625]
Reactivity:
Rat
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.072 ng/mL
Sizes Available: 48 T, 96 T
Rat TPH2 ELISA Kit [orb442692]
Reactivity:
Rat
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.072 ng/mL
Sizes Available: 48 T, 96 T
Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit [orb1663058]
Reactivity:
Rat
Sizes Available: 96 T, 48 T