Size : 1mLx10mM(inDMSO)
Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl
| Description | TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA. |
| Targets & IC50 | MCF-7 cells:> 50 μM (GI50), WI-38 cells:> 50 μM (GI50), NS3/4A:12.8 μM, A549 cells:> 50 μM (GI50) |
| In vitro | The growth inhibitory activity (GI₅₀) of TCEP hydrochloride in A549, MCF-7 tumor cells and WI-38 normal cells was all greater than 50 μM.[1] |
| In vivo | TCEP hydrochloride can dose-dependently promote NF-κB-DNA binding within the concentration range of 0.25–6 mM, and can replace DTT for in vitro NF-κB binding experiments. Compared with DTT, TCEP hydrochloride does not significantly weaken the inhibitory effect of Hg^2+ on NF-κB-DNA binding. When 6 mM TCEP hydrochloride is present, 20 μM Hg^2+ can effectively block NF-κB-DNA binding, while at 0.25 mM DTT conditions, more than 100 μM Hg^2+ is required to achieve a similar effect. [2] TCEP hydrochloride can be used to reduce the disulfide bonds in hLF peptides. At a peptide concentration of 0.1 mM, adding 30-fold molar excess of TCEP hydrochloride and incubating at 37 ℃ for 2 hours can achieve effective reduction. [3] |
| Synonyms | Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl |
| Molecular Weight | 286.65 |
| Formula | C9H16ClO6P |
| Cas No. | 51805-45-9 |
| Smiles | Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O |
| Relative Density. | 1.041 g/cm3 at 25℃ |
| Storage | Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 50 mg/mL (174.43 mM), Sonication is recommended.  DMSO: 252.5 mg/mL (880.87 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+90% Saline: 2.75 mg/mL (9.59 mM), Solution. 5% DMSO+95% Saline: 1.45 mg/mL (5.06 mM), Solution. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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