Tissue Inhibitors of Metalloproteinase 4 (TIMP-4) BioAssay™ ELISA Kit (Human)

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Serum, plasma, tissue homogenates, human milk

For the quantitative determination of human tissue inhibitors of metalloproteinase 4 (TIMP-4) concentrations in serum, plasma, tissue homogenates.

0.005ng/ml

0.313-20ng/ml

This assay has high sensitivity and excellent specificity for detection of human TIMP-4. No significant cross-reactivity or interference between human TIMP-4 and analogues was observed.

This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Assay plate (12x8 coated microwells), 1x96 wells
Standard (Freeze dried), 2x vials
Biotin-antibody (100X), 1x120ul
HRP-avidin (100X), 1x120ul
Biotin-antibody Diluent, 1x15ml
HRP-avidin Diluent, 1x15ml
Sample Diluent, 1x50ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml
Adhesive Strip (For 96 wells), 4x strips
Instruction manual

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.