Tissue Inhibitors of Metalloproteinase 2 (TIMP-2) BioAssay™ ELISA Kit (Mouse)

TIMP-2 gene is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22kD and 194aa.

The Tissue Inhibitors of Metalloproteinase 2 (TIMP-2) BioAssay™ ELISA Kit is for the quantitative detection of mouse TIMP-2 in cell culture supernates, serum and plasma (heparin, EDTA).

156-10000pg/ml

<10pg/ml

Natural and recombinant mouse TIMP-2

This mouse TIMP-2 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-2 has been precoated onto 96-well plates. Standards(NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-2 amount of sample captured in plate.

353077A: Microtiter Strips, 1x96 wells
*353077B: TIMP-2 Standard, 2x10ng
353077C: Anti-mouse TIMP-2 (Biotin), 1x100ul (1:100 dilution)
143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x100ul (1:100 dilution)
143697: Sample Diluent Buffer, 1x30ml
143698: Antibody Diluent Buffer, 1x12ml
143699: ABC Diluent Buffer, 1x12ml
143700: TMB Color Developing Agent, 1x10ml
143701: TMB Stop Solution, 2N H2SO4, 1x10ml
143702: Wash Buffer, 25X, 1x20ml

Store *353077B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.