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> Tween 20 [9005-64-5]

Tween 20 [9005-64-5]

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Cat# HY-141415-100mL

Size : 100mL

Marca : MedChemExpress


Description

Tween 20 (Polysorbate 20) is a polyoxyethylene (POE)-type nonionic surfactant[1][2][3].

In Vitro

Addition of Tween 20 can modify lignin surface properties to change its hydrophobicity, hydrogen bonding ability and surface charges, thus reducing the non-productive adsorption of proteins[1].
Tween 20 (0.35%-0.7% w/w) added to 0.05 mol/L of phosphate buffer pH 6.5 dramatically increased the apparent solubility of the drug from 0.8 μg/ml even to 353 μg/ml, while its effect in lower pH or in water was much smaller (20 μg/ml in pH 4.5). The increased concentration of phosphate salts (0.2 mol/l) at pH 6.5 in the presence of 0.7% of Tween 20, resulted in further increase of candesartan cilexetil solubility to 620 μg/ml[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tween 20 Related Antibodies

Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.67 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureté et documentation
Références