Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Urea (BUN) Colorimetric Assay Kit (Urease Method)

Stampa

Cat# E-BC-K183-S-50

Size : 50Assays

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : Elabscience

Contatta il distributore locale :

Telefono : +1 850 650 7790

Detection Principle	Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with phenol chromogenic agent and form a blue substance in alkaline medium, and the production of the blue substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm.
Synonyms	BUN
Sample Type	Serum,Plasma,Urine,Saliva,Milk
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (580 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.114 mmol/L
Detection Range	0.114-30 mmol/L
Precision	inter-assay CV: 4.7% \| intra-assay CV: 4.6%
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K016-S	Uric Acid (UA) Colorimetric Assay Kit	100Assays , 50Assays
E-BC-K329-S	Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Rabbit plasma	1
Rat serum	1
Rat plasma	1
Human serum	1
Human saliva	1
Human milk	1
Human urine	30-60
Mouse urine	30-60

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.