ASM10221-STR-100 | GABA-A Receptor Delta Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (ASM10221). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (ASM10221) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.
Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (ASM10221). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (ASM10221) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF, AM
Primary Accession	P18506
Other Accession	NP_058985.1
Host	Mouse
Isotype	IgG2a
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat GABA-A Receptor Delta Monoclonal IgG2a
Target/Specificity	Detects ~55kDa.
Other Names	GABAA-RD Antibody, Gabrd Antibody, MGC105467 Antibody
Clone Names	S151-3
Immunogen	Synthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Membrane \| Cell Junction \| Synapse \| Postsynaptic Cell Membrane

GABA-A Receptor Delta Antibody