ASM10271-STR-100 | Synaptotagmin-12 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (ASM10271). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (ASM10271) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-12 Antibody (D) Composite.
Western Blot analysis of Monkey COS cells transfected with GFP-tagged Synaptotagmin showing detection of ~45 kDa Synaptotagmin-12 protein using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (ASM10271). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Synaptotagmin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (ASM10271) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~45 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF
Primary Accession	Q920N7
Other Accession	NP_598925.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Mouse Synaptotagmin-12 Monoclonal IgG1
Target/Specificity	Detects ~45kDa. Does not cross-react with Synaptotagmin-6 or other Synaptotagmins.
Other Names	SYT-12 Antibody, SYT12 Antibody, Synaptotagmin 12 Antibody, Synaptotagmin XII Antibody, SytXII Antibody, Syt XII Antibody, Synaptotagmin12 Antibody, SynaptotagminXII Antibody
Clone Names	S277-7
Immunogen	Fusion protein amino acids 168-255 (Cytoplasmic C2A domain) of mouse Synaptotagmin-12
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-437 was sufficient for detection of Synaptotagmin-12 in 20 µg of transiently overexpressing synaptotagmin-12 COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasmic Vesicle

Synaptotagmin-12 Antibody