Ascorbic Acid Assay Kit, BioAssay™

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. This Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample. This kit is suitable for the determination of ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates.

Sample size: 20ul

Colorimetric Assay: 6 to 1000uM ascorbic acid
Fluorometric Assay: 1 to 100uM ascorbic acid

Assays: Ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates
Drug Discovery/Pharmacology: Effects of drugs on ascorbic acid metabolism

A3774-61A: Enzyme Mix, 1x120ul
A3774-61B: Standard, 1x400ul. 10mM Ascorbic acid
A3774-61C: Assay Buffer, 1x10ml
A3774-61D: Dye Reagent, 1x120ul

Optical density plate reader
Fluorescence plate reader.
Pipeting devices
Centrifuge tubes
Clear flat-bottom uncoated 96-well plates
Black flat-bottom uncoated 96-well plates

Store all kit components at --20°C. Kit is stable for 6 months.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.