Human ACO1 (Cytoplasmic aconitate hydratase) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	ACO1
Gene ID:	48
Uniprot ID:	ACOHC_HUMAN
Specificity:	This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
Protein Name	Cytoplasmic Aconitate Hydratase Aconitase Citrate Hydro-Lyase Ferritin Repressor Protein Iron Regulatory Protein 1 Irp1 Iron-Responsive Element-Binding Protein 1 Ire-Bp 1
Database Links	Reactome: R-HSA-917937
Cellular Localisation	Cytoplasm Cytosol
Alternative CLIA Names	Cytoplasmic Aconitate Hydratase CLIA kit Aconitase CLIA kit Citrate Hydro-Lyase CLIA kit Ferritin Repressor Protein CLIA kit Iron Regulatory Protein 1 CLIA kit Irp1 CLIA kit Iron-Responsive Element-Binding Protein 1 CLIA kit Ire-Bp 1 CLIA kit ACO1 CLIA kit IREB1 CLIA kit
Specificity	This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-106	100
EDTA plasma(n=8)	98-110	104
Cell culture media(n=8)	92-105	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	106.87	286.48	886.35	115.85	276.99	826.38
Standard deviation	11.42	28.39	70.64	12.91	25.46	50.24
CV (%)	10.69	9.91	7.97	11.14	9.19	6.08

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance