Human ANGPTL3 (Angiopoietin-related protein 3) CLIA Kit

Référence NB-22-40406-1

Conditionnement : 96T

Marque : Neo Biotech

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  • Human ANGPTL3 (Angiopoietin-related protein 3) CLIA Kit

General Info

Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human ANGPTL3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: ANGPTL3
Gene ID: 27329
Uniprot ID: ANGL3_HUMAN
Specificity: This kit recognizes Human ANGPTL3 in samples. No significant cross-reactivity or interference between Human ANGPTL3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Tissue Specificity Expressed principally in liver. Weakly expressed in kidney. Binds to adipocytes. Increased expression and colocalization with activated ITGB3 in glomeruli of patients with nephrotic syndrome showing effaced podocyte foot processes (at protein level).
Post Translational Modifications O-glycosylated at Thr-226 by GALNT2.blocks processing and activation by proprotein convertases. In part proteolytically cleaved by proprotein convertases.proposed to be involved in activation.
Function Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface.the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids. Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol. Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food.the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT. Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity. ANGPTL3(17-221): In vitro inhibits LPL activity.not effective on GPIHBP1-stabilized LPL. Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration. Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling. May increase the motility of podocytes. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS.
Protein Name Angiopoietin-Related Protein 3
Angiopoietin-5
Ang-5
Angiopoietin-Like Protein 3 Cleaved Into - Angptl3(17-221 - Angptl3(17-224
Database Links Reactome: R-HSA-8963889
Reactome: R-HSA-9029558
Cellular Localisation Secreted
Cell Projection
Lamellipodium
Colocalized With Hspg2 And Activated Itgb3 On Podocytes
Alternative CLIA Names Angiopoietin-Related Protein 3 CLIA kit
Angiopoietin-5 CLIA kit
Ang-5 CLIA kit
Angiopoietin-Like Protein 3 Cleaved Into - Angptl3(17-221 - Angptl3(17-224 CLIA kit
ANGPTL3 CLIA kit
ANGPT5 CLIA kit
UNQ153 CLIA kit
PRO179 CLIA kit
Specificity This kit recognizes Human ANGPTL3 in samples. No significant cross-reactivity or interference between Human ANGPTL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma(n=8) 96-112 102
Cell culture media(n=8) 97-113 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 96.97 189.42 758.89 98.73 196.08 793.85
Standard deviation 11.37 13.64 86.06 8.12 21.39 85.50
CV (%) 11.73 7.20 11.34 8.22 10.91 10.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance