General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This human ANGPTL6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | ANGPTL6 |
| Gene ID: | 83854 |
| Uniprot ID: | ANGL6_HUMAN |
| Specificity: | This kit recognizes Human ANGPTL6 in samples. No significant cross-reactivity or interference between Human ANGPTL6 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure. |
| Protein Name | Angiopoietin-Related Protein 6Angiopoietin-Like Protein 6Angiopoietin-Related Growth FactorAngiopoietin-Related Protein 5 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Angiopoietin-Related Protein 6 CLIA kitAngiopoietin-Like Protein 6 CLIA kitAngiopoietin-Related Growth Factor CLIA kitAngiopoietin-Related Protein 5 CLIA kitANGPTL6 CLIA kitAGF CLIA kitARP5 CLIA kitUNQ152 CLIA kitPRO178 CLIA kit |
| Specificity | This kit recognizes Human ANGPTL6 in samples. No significant cross-reactivity or interference between Human ANGPTL6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 101-112 | 106 |
| EDTA plasma(n=8) | 85-96 | 91 |
| Cell culture media(n=8) | 101-116 | 107 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.11 | 1.80 | 9.65 | 1.09 | 1.66 | 8.79 |
| Standard deviation | 0.10 | 0.19 | 0.98 | 0.09 | 0.19 | 0.76 |
| CV (%) | 9.01 | 10.56 | 10.16 | 8.26 | 11.45 | 8.65 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance