General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This human ACHE kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | ACHE |
| Gene ID: | 43 |
| Uniprot ID: | ACES_HUMAN |
| Specificity: | This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Isoform H is highly expressed in erythrocytes. |
| Function | Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis. |
| Protein Name | AcetylcholinesteraseAche |
| Database Links | Reactome: R-HSA-112311Reactome: R-HSA-1483191Reactome: R-HSA-422085 |
| Cellular Localisation | SynapseSecretedCell MembranePeripheral Membrane ProteinIsoform T: NucleusOnly Observed In Apoptotic NucleiIsoform H: Cell MembraneLipid-AnchorGpi-AnchorExtracellular Side |
| Alternative CLIA Names | Acetylcholinesterase CLIA kitAche CLIA kitACHE CLIA kit |
| Specificity | This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-97 | 91 |
| EDTA plasma(n=8) | 94-107 | 101 |
| Cell culture media(n=8) | 85-96 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.09 | 2.33 | 9.79 | 1.15 | 2.27 | 9.26 |
| Standard deviation | 0.12 | 0.25 | 0.93 | 0.11 | 0.16 | 1.06 |
| CV (%) | 11.01 | 10.73 | 9.50 | 9.57 | 7.05 | 11.45 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance