Rat CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit

Référence NB-22-41806-1

Conditionnement : 96T

Marque : Neo Biotech

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  • Rat CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit

General Info

Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pmol/mL
Detection Limit: 12.5~800pmol/mL
Short Description: This rat CHRM2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: Chrm2
Gene ID: 81645
Uniprot ID: ACM2_RAT
Specificity: This kit recognizes Rat CHRM2 in samples. No significant cross-reactivity or interference between Rat CHRM2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Post Translational Modifications Phosphorylated in response to agonist treatment.
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3).this then triggers calcium ion release into the cytosol.
Protein Name Muscarinic Acetylcholine Receptor M2
Database Links Reactome: R-RNO-390648
Reactome: -RNO-418594
Reactome: -RNO-8856825
Reactome: -RNO-8856828
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Phosphorylation In Response To Agonist Binding Promotes Receptor Internalization
Alternative CLIA Names Muscarinic Acetylcholine Receptor M2 CLIA kit
Chrm2 CLIA kit
Chrm-2 CLIA kit
Specificity This kit recognizes Rat CHRM2 in samples. No significant cross-reactivity or interference between Rat CHRM2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-110 104
EDTA plasma(n=8) 90-102 96
Cell culture media(n=8) 94-104 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pmol/mL) 42.32 106.40 276.89 43.92 109.77 267.14
Standard deviation 3.56 8.83 30.60 3.92 10.81 26.05
CV (%) 8.41 8.30 11.05 8.93 9.85 9.75
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance