Conditionnement : 100assays
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Histone acetyltransferase (HAT) is an enzyme that acetylates conserved lysine on a histone protein by transferring an acetyl group from acetyl CoA. Histone acetylation can increase gene expression. HAT plays important roles in gene transcription, cell proliferation and differentiation.
The HAT Activity Colorimetric Assay Kit provides a fast and sensitive way for detection of HAT activity in mammalian samples based on colorimetric method that eliminates radioactivity in traditional assays. The assay utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. The active HAT acetylates peptide substrate and then releases the free form of CoA which then acts as an essential coenzyme for generating NADH. NADH can be easily detected upon reacting with a soluble tetrazolium dye using a spectrophotometer or an ELISA plate reader. The detection can be continuous and suited for kinetic studies.