SOD Activity Assay Kit

Référence CA061

Conditionnement : 100assays

Marque : Canvax Biotech

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

SOD Activity Assay Kit

For an Easy & Fast Quantitative determination of Superoxide Dismutase (SOD) enzyme activity

Superoxide dismutases (SOD) catalyse the breakdown of superoxide radicals and provide the first line of defense against oxygen toxicity.

SOD Assay Kit, superoxide ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by Xanthine Oxidase (XO). The superoxide anion then coverts the tetrazolium salt WST-1 to the colored product WST-1 formazan.

Absorbance is then measured at 450 nm using a standard microplate reader. Addition of SOD to this reaction reduces superoxide ion levels, thereby lowering the rate of WST-1 formazan formation. SOD activity in the experimental sample is measured as the percent inhibition of the rate of WST-1 formazan formation.

100 assays
SKU: CA061 Categories: Cell Based Assays, Stress Oxidative

Detailed information:

  • Easy-to-use.
  • Fast protocol.

– 1 mL WST-1 Reagent
– 20 mL SOD assay buffer
– 10 mL SOD dilution buffer
– 30 µL SOD enzyme solution
– 1 mg SOD standard (3000 U/mg)

Datasheet
MSDS
  • Quantitative determination of Superoxide Dismutase (SOD) enzyme activity.

t

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
  • Shipping: Gel pack.
  • Storage: The SOD Standard should be stored at ‐20 °C. All other components of this kit are stable at 4 °C until the kit expiration date.
  • Recommendations: WST-1 reagent is light sensitive and should be maintained in amber tubes.
  • López Vázquez, C. (2023). Effects of symbiotic N fixation on N and water use efficiency in common bean plants: physiological, molecular and biochemical characterization.
  • Alshehade, S. A., Al Zarzour, R. H., Mathai, M., Giribabu, N., Seyedan, A., Kaur, G., … & Alshawsh, M. A. (2023). Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic–Oleic Acid-Induced Steatosis in HepG2 Cells. Pharmaceuticals16(1), 109.
  • Alshehade, S. A., Al Zarzour, R. H., Murugaiyah, V., Lim, S. Y. M., El-Refae, H. G., & Alshawsh, M. A. (2022). Mechanism of Action of Orthosiphon stamineus against Non-Alcoholic Fatty Liver Disease: Insights from Systems Pharmacology and Molecular Docking Approaches. Saudi Pharmaceutical Journal.
  • García-Sanmartín, J., Bobadilla, M., Mirpuri, E., Grifoll, V., Pérez-Clavijo, M., & Martínez, A. (2022). Agaricus Mushroom-Enriched Diets Modulate the Microbiota-Gut-Brain Axis and Reduce Brain Oxidative Stress in Mice. Antioxidants11(4), 695.
  • Bobadilla, Miriam, et al. “A Grape Juice Supplemented with Natural Grape Extracts Is Well Accepted by Consumers and Reduces Brain Oxidative Stress.” Antioxidants 10.5 (2021): 677.
  • Bobadilla, M., García-Sanmartín, J., & Martínez, A. (2021). Natural Food Supplements Reduce Oxidative Stress in Primary Neurons and in the Mouse Brain, Suggesting Applications in the Prevention of Neurodegenerative Diseases. Antioxidants10(1), 46.
  • López, C. M., Pineda, M., & Alamillo, J. M. (2020). Differential Regulation of Drought Responses in Two Phaseolus vulgaris Genotypes. Plants9(12), 1815.
  • Gallego, P., Castejón-Vega, B., del Campo, J. A., & Cordero, M. D. (2020). The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging. Cells9(10), 2148.
  • Lima-Cabello, E., Morales-Santana, S., León, J., Alché, V., Clemente, A., de Dios Alche, J., & Jimenez-Lopez, J. C. (2018). Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse back the induced insulin resistance in pancreatic cells. Food & Function.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT