TACE Activity, Fluorometric 520, BioAssay™ Kit (alpha-Secretase, ADAM17, A Disintegrin and Metalloprotease)

The TACE Activity Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of TACE inhibitors. It contains a 5-FAM FRET substrate, derived from a sequence surrounding the cleavage site of TACE. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by a TACE Inhibitor. Active TACE cleaves FRET substrate into two separate fragments resulting in an increase of 5-FAM fluorescence which can be monitored at excitation/emission = 490nm/520nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of cell components and test compounds.

T0500-12A: TACE substrate (4mM), 1x50ul
T0500-12B: Fluoresence Standard (5-FAM), 1mM, 1x10ul
T0500-12C: Assay Buffer, 1x20ml
T0500-12D: TACE Inhibitor (1mM), 1x10ul
T0500-12E: Stop Solution, 1x10ml

Store all kit components at -20°C. Protect T0500-12A and T0500-12B from light and from moisture. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.