10× Transcription Buffer, GMP Grade
Referentie GMP-EB231-M010
Formaat : 10ml
Merk : Novoprotein
Description 10×Transcription Buffer is the companion reaction buffer for T7 RNA polymerase IVT. This buffer is optimized to transcribe 150-200μg of RNA in a single reaction, and the synthesized RNA can be used in many aspects such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. downstream applications.
Features - Versatility: suitable for in vitro RNA synthesis reactions;
- Efficiency: This system can obtain 150-200μg RNA in one transcription reaction.
Quality Control | Element | Standard |
|---|---|
| Appearance | Clear and transparent solution |
| Visible Particles | Meet the specification |
| pH | 7.5-8.5 |
| Endonuclease Residues | The degradation of substrate was ≤10% |
| Exonuclease Residues | The degradation of substrate was ≤10% |
| RNase Residues | The degradation of substrate was ≤10% |
| Bacterial Endotoxins | ＜1EU/ml |
| Mycoplasma | Negative |
| Heavy Metals | ≤10ppm |
| Microbial Limit | Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml |
Storage At -20±5℃.
Components | SKU | Components | Volume |
|---|---|---|
| GMP-EB231-M001 | 10×Transcription Buffer, GMP Grade | 1ml |
| GMP-EB231-M005 | 10×Transcription Buffer, GMP Grade | 5ml |
| GMP-EB231-M010 | 10×Transcription Buffer, GMP Grade | 10ml |
| GMP-EB231-M050 | 10×Transcription Buffer, GMP Grade | 50ml |
Related Products | Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| GMP-M062 | Vaccinia Capping Enzyme, GMP Grade | GMP-E125 | RNase Inhibitor, GMP Grade |
| GMP-M072 | mRNA Cap 2´-O-Methyltransferase, GMP Grade | GMP-E127 | DNase I, GMP Grade |
| GMP-M012 | E. coli Poly(A) Polymerase, GMP Grade | GMP-M036 | Pyrophosphatase, Inorganic (yeast), GMP Grade |
| GMP-E121 | T7 RNA Polymerase, GMP Grade | GMP-E131 | T7 RNA Transcription Enzyme Mix, GMP Grade |
| GMP-S023-026 | NTPs, GMP Grade | ||