2X hyperfusion plus master mix (With dye)

Our product 2X Hyperfusion plus master mix (With dye) provides superior performance for major PCR applications with high fidelity, powerful amplification ability and faster extension speed. Hyperfusion plus DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes hyperfusion plus DNA polymerase a superior choice for detection or other subsequent applications. Hyperfusion plus DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu).

Hyperfusion plus DNA polymerase possesses 5´→3´ polymerase activity and 3´→5´ exonuclease activity. It will generate blunt-ended products in the amplification products without an A overhang which appears in the product amplified with Taq polymerase. The polymerase has been capable of amplifying genomic fragment as long as 20.1 kb in our assays. In PCR reaction, elongation rate of hyperfusion plus DNA polymerase is about 15-30 sec/kb depending on the complexity of the amplicon.

2X Hyperfusion plus master mix (With dye) is a ready-to-use 2×premix solution containing polymerase, dNTPs, an already optimized buffer system and dye. Our mix is ideal for any detection and the PCR product can be directly electrophoresed after the amplification without the need to add a loading buffer.

If the subsequent experiment is cloning, product 2X Hyperfusion plus master mix (Cat. No K1119) is your better choice which doesn’t contain a dye.