Product Info Summary
| SKU: | AR1068 |
|---|---|
| Size: | 500mL |
Product info
General Description
Paraformaldehyde (PFA) is the polymerization product of formaldehyde with a typical degree of polymerization of 8–100 units. Paraformaldehyde is not a fixative itself; it must be depolymerized to formaldehyde in solution. In cell culture, typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. Fixing ensures that sample cell structures stay intact and that antigens are immobilized, while ideally still permitting unfettered access of antibodies to target antigens.
Our 4% Paraformaldehyde is the most preferred fixative agent as it builds covalent cross-links between molecules. This glues them together hence effectively preserving cells and tissue components. Use of this 4% paraformaldehyde can guarantee consistency in the physical and chemical properties of the cell, hence no change in chemical and morphology characteristics of the cells and tissues.
Since the 4% paraformaldehyde is not fixative itself, it is required that formaldehyde is freshly prepared from the PFA stock.
Overview
| Product Name | 4% Paraformaldehyde (PFA) Solution in PBS (with DEPC) |
|---|---|
| SKU/Catalog Number | AR1068 |
| Form | Liquid |
| Physical State | The solution should be clear, colorless, with no precipitate. |
| Pack Size | 500mL |
| Application | Preserving Samples, Fixing Cells For Histology *Our Boster Guarantee covers the use of this product in the above tested applications. |
| Storage | Store at -20˚C for one year. |
| Cite This Product | 4% Paraformaldehyde (PFA) Solution in PBS (with DEPC) (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1068) |
| Equivalent | N/A |
Properties
| CAS Number | 30525-89-4 |
|---|---|
| MDL Number | MFCD00133991 |
| Solubility | Soluble in water |
| pH | 7.0 - 7.6 at 25°C |
Safety and Handling
•Causes serious eye damage .
• Suspected of causing cancer.
• May cause an allergic skin reaction.
• Causes skin irritation.
• Avoid breathing dust/fume/gas/mist/vapors/spray.
• Wear protective gloves/protective clothing/eye protection/face protection.
•IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• Store locked up.
•Dispose of contents/container in accordance with local/regional/national/international regulations.
General Notes
• Adjust pH to 7.2-7.6 with NaH2PO4 if pH <7 after long storage.
• Deposition is normal for this product.
4% Paraformaldehyde Recipe
To prepare a solution of 4% paraformaldehyde you need to use this protocol. The following ingredients are required:
•1M Sodium Hydroxide
•10X phosphate-buffered saline (PBS)
•1M Hydrochloric acid
Note: This should be done in a fume chamber with gloves, safety glasses, and a face mask.
1. To prepare a 4% of paraformaldehyde (PFA) solution in PBS, add 4 grams of granular or prill paraformaldehyde to water (H2O)
2. Use stirring hot plate to heat the solution to 60-degree celsius. Add 1mL of 1M NaOH until the paraformaldehyde is fully dissolved.
3. Add 10 mL of 10x phosphate-buffered saline (PBS) and allow the mixture to cool.
4. Adjust the solution pH with 1M HCL then top up the volume to 100 mL with H2O.
5. Filter the solution to remove any deposit.
Question
Can AR1068 be used for electron microscopy biological sample preparation?
Verified customer
Asked: 2020-10-19
Answer
The 4% Paraformaldehyde (PFA) Solution In PBS (AR1068) can not be used directly for electron microscopy. It could mix with other reagents such as glutaraldehyde to be used for electron microscopy.
Boster Scientific Support
Answered: 2020-10-20