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5-Ethynyl-2'-deoxyuridine [61135-33-9]

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Referentie NB-64-08276-100mg

Formaat : 100mg

Merk : Neo Biotech

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5-Ethynyl-2'-deoxyuridine

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Synonyms:

Catalog No. T17341 Copy Product Info

Purity: 99.04%

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine used to monitor de novo DNA synthesis through click chemistry and serves as an alkyl chain-based PROTAC linker for synthesizing PROTACs.

Cas No. 61135-33-9

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.04%

Color:White to Yellow

Product Introduction

Bioactivity

Description	5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine used to monitor de novo DNA synthesis through click chemistry and serves as an alkyl chain-based PROTAC linker for synthesizing PROTACs.
In vitro	PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].Like 5-bromo-2’-deoxyuridine (BrdU), EdU is incorporated into DNA during active DNA synthesis. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.Modified nucleosides, including EdU, can be cytotoxic when added to cells for prolonged periods[2].5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[3]

Chemical Properties

Molecular Weight	252.22
Formula	C₁₁H₁₂N₂O₅
Cas No.	61135-33-9
Smiles	OC[C@H]1O[C@H](C[C@@H]1O)n1cc(C#C)c(=O)[nH]c1=O
Relative Density.	1.55g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 8 mg/mL (31.72 mM), Sonication is recommended.

DMSO: 130 mg/mL (515.42 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9648 mL	19.8240 mL	39.6479 mL	198.2396 mL
5 mM	0.7930 mL	3.9648 mL	7.9296 mL	39.6479 mL
10 mM	0.3965 mL	1.9824 mL	3.9648 mL	19.8240 mL
20 mM	0.1982 mL	0.9912 mL	1.9824 mL	9.9120 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0793 mL	0.3965 mL	0.7930 mL	3.9648 mL
100 mM	0.0396 mL	0.1982 mL	0.3965 mL	1.9824 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

PROTACLinkerPROTAC LinkersPROTAC LinkerInhibitorinhibitAlkyl-Chain5Ethynyl2'deoxyuridine5-Ethynyl-2'-deoxyuridine5 Ethynyl 2' deoxyuridine

Related Tags: 5-Ethynyl-2'-deoxyuridine chemical structure | 5-Ethynyl-2'-deoxyuridine in vitro | 5-Ethynyl-2'-deoxyuridine formula | 5-Ethynyl-2'-deoxyuridine molecular weight